Test-Retest Repeatability of [¹⁸F]MC225-PET in Rodents: A Tracer for Imaging of P-gp Function.

ABSTRACT: In longitudinal PET studies, animals are repeatedly anesthetized which may affect the repeatability of PET measurements. The aim of this study was to assess the effect of anesthesia on the P-gp function as well as the reproducibility of [¹⁸F]MC225 PET scans. Thus, dynamic PET scans with blood sampling were conducted in 13 Wistar rats. Seven animals were exposed to isoflurane anesthesia 1 week before the PET scan ("Anesthesia-exposed" PET). A second group of six animals was used to evaluate the reproducibility of measurements of P-gp function at the blood-brain barrier (BBB) with [¹⁸F]MC225. In this group, two PET scans were made with a 1 week interval ("Test" and "Retest" PET). Pharmacokinetic parameters were calculated using compartmental models and metabolite-correc

SUBMITTER: Garcia-Varela L 

PROVIDER: S-EPMC7034080 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature