Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration.

ABSTRACT:

Objectives

The heterogeneity of meniscus cells and the mechanism of meniscus degeneration is not well understood. Here, single-cell RNA sequencing (scRNA-seq) was used to identify various meniscus cell subsets and investigate the mechanism of meniscus degeneration.

Methods

scRNA-seq was used to identify cell subsets and their gene signatures in healthy human and degenerated meniscus cells to determine their differentiation relationships and characterise the diversity within specific cell types. Colony-forming, multi-differentiation assays and a mice meniscus injury model were used to identify meniscus progenitor cells. We investigated the role of degenerated meniscus progenitor (DegP) cell clusters during meniscus degeneration using computational analysis and experimental ve

SUBMITTER: Sun H 

PROVIDER: S-EPMC7034356 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature