Tight DNA-protein complexes isolated from barley seedlings are rich in potential guanine quadruplex sequences.

ABSTRACT:

Background

The concept of chromatin domains attached to the nuclear matrix is being revisited, with nucleus described as a set of topologically associating domains. The significance of the tightly bound to DNA proteins (TBP), a protein group that remains attached to DNA after its deproteinization should be also revisited, as the existence of these interactions is in good agreement with the concept of the topologically associating domain. The work aimed to characterize the DNA component of TBP isolated from barley seedlings.

Methods

The tight DNA-protein complexes from the first leaves, coleoptiles, and roots of barley seedlings were isolated by purification with chromatography on nitrocellulose or exhaustive digestion of DNA with DNase I. Cloning and transformation were perf

SUBMITTER: Sjakste T 

PROVIDER: S-EPMC7034378 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature