Myeloid atg5 deletion impairs n-3 PUFA-mediated atheroprotection.

ABSTRACT:

Background and aims

Dietary long-chain (≥20 carbons) n-3 polyunsaturated fatty acids (PUFAs) reduce atherosclerosis and enhance macrophage autophagy activation. How macrophage autophagy impacts atherosclerotic progression, particularly when comparing dietary n-3 PUFA supplementation vs. saturated fat feeding, is unknown.

Methods

We generated myeloid-specific autophagy-deficient and control mice in the Ldlr^-/- background by transplanting bone marrow from myeloid-specific autophagy-related (atg) 5 knockout mice and wild type controls into irradiated Ldlr^-/- recipients. After 7 weeks for recovery from radiation, mice were fed an atherogenic diet containing 0.2% cholesterol and 20% calories as palm oil (PO diet), or 10% calories as PO plus 10% calories as f

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7034781 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature