Host factor prioritization for pan-viral genetic perturbation screens using random intercept models and network propagation.

ABSTRACT: Genetic perturbation screens using RNA interference (RNAi) have been conducted successfully to identify host factors that are essential for the life cycle of bacteria or viruses. So far, most published studies identified host factors primarily for single pathogens. Furthermore, often only a small subset of genes, e.g., genes encoding kinases, have been targeted. Identification of host factors on a pan-pathogen level, i.e., genes that are crucial for the replication of a diverse group of pathogens has received relatively little attention, despite the fact that such common host factors would be highly relevant, for instance, for devising broad-spectrum anti-pathogenic drugs. Here, we present a novel two-stage procedure for the identification of host factors involved in the replication of dif

SUBMITTER: Dirmeier S 

PROVIDER: S-EPMC7034926 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature