Catalysis and Electron Transfer in De Novo Designed Helical Scaffolds.

ABSTRACT: The relationship between protein structure and function is one of the greatest puzzles within biochemistry. De novo metalloprotein design is a way to wipe the board clean and determine what is required to build in function from the ground up in an unrelated structure. This Review focuses on protein design efforts to create de novo metalloproteins within alpha-helical scaffolds. Examples of successful designs include those with carbonic anhydrase or nitrite reductase activity by incorporating a ZnHis₃ or CuHis₃ site, or that recapitulate the spectroscopic properties of unique electron-transfer sites in cupredoxins (CuHis₂ Cys) or rubredoxins (FeCys₄ ). This work showcases the versatility of alpha helices as scaffolds for metalloprotein design and

SUBMITTER: Pinter TBJ 

PROVIDER: S-EPMC7035182 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature