Characterization of variations within the rumen metaproteome of Holstein dairy cattle relative to morning feed offering.

ABSTRACT: Few studies have utilized proteomic techniques to progress our knowledge of protein-mediated pathways within the rumen microbial community, and no previous research has used these techniques to investigate the patterns or variations of these proteins within this community. It was hypothesized that there would be fluctuations of rumen microbial protein abundances due to feed intake-mediated nutrient availability and that these could be identified using non gel-based proteomic techniques. This study investigated the fluctuations of bovine rumen metaproteome utilizing three mid to late-lactation Holsteins. Rumen fluid was collected at three timepoints on three days relative to their first morning feed offering (0 h, 4 h, and 6 h). Samples were pooled within timepoint within cow across day, an

SUBMITTER: Honan MC 

PROVIDER: S-EPMC7035244 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature