CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition.

ABSTRACT: OBJECTIVES:The laminins (LM) are a family of basement membranes glycoproteins with essential roles in supporting epithelia, endothelia, nerves and muscle adhesion, and in regulating a range of processes including cell migration, stem cell maintenance and differentiation. However, surprisingly little is known about the mechanisms of turnover and remodelling of LM networks due to lack of appropriate tools to study these processes at the necessary resolution. Recently, the nematode C. elegans ortholog of human the LMβ1 chain was labelled at the C-terminus with the photoconvertible fluorophore Dendra2. Here we used genome editing to establish a similar system in a mammalian cell line as proof of concept for future mammalian models. RESULTS:CRISPR-Cas9 was used to introduce the Dendra2 sequence

SUBMITTER: Shaw L 

PROVIDER: S-EPMC7035763 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature