Systematic identification of long intergenic non-coding RNAs expressed in bovine oocytes.

ABSTRACT:

Background

Long non-coding RNAs (lncRNAs) are key regulators of diverse cellular processes. Although a number of studies have reported the identification of bovine lncRNAs across many tissues, very little is known about the identity and characteristics of lncRNAs in bovine oocytes.

Methods

A bovine oocyte cDNA library was constructed and sequenced using the Illumina HiSeq 2000 sequencing system. The oocyte transcriptome was constructed using the ab initio assembly software Scripture and Cufflinks. The assembled transcripts were categorized to identify the novel intergenic transcripts, and the coding potential of these novel transcripts was assessed using CPAT and PhyloCSF. The resulting candidate long intergenic non-coding RNAs (lincRNAs) transcripts were further evaluated t

SUBMITTER: Wang J 

PROVIDER: S-EPMC7035783 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature