A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy.

ABSTRACT:

Background

Induced pluripotent stem cells (iPSCs) exhibit limitless pluripotent plasticity and proliferation capability to provide an abundant cell source for tissue regenerative medicine. Thus, inducing iPSCs toward a specific differentiation direction is an important scientific question. Traditionally, iPSCs have been induced to chondrocytes with the help of some small molecules within 21-36 days. To speed up the differentiation of iPSCs, we supposed to utilize bioactive ceramics to assist chondrogenic-induction process.

Methods

In this study, we applied ionic products (3.125~12.5 mg/mL) of the lithium-containing bioceramic (Li₂Ca₄Si₄O₁₃, L2C4S4) and individual Li⁺ (5.78~23.73 mg/L) in the direct chondrogenic different

SUBMITTER: Hu Y 

PROVIDER: S-EPMC7035784 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature