Mutant dlx3b disturbs normal tooth mineralization and bone formation in zebrafish.

ABSTRACT:

Background

Tricho-dento-osseous (TDO) syndrome is an autosomal dominant disorder characterized by anomalies in hair, teeth and bone (OMIM190320). Various mutations of Distal-Less 3 (DLX3) gene are found to be responsible for human TDO. The aim of this study was to investigate effects of DLX3 on tooth and bone development using a zebrafish model.

Methods

The dlx3b mutant zebrafish lines were established using the gene targeting tool transcription activator-like effector nuclease (TALEN). Micro-computed tomography was used to render the three-dimensional skeletal structures of mutant fishes. The pharyngeal bone along with connected teeth was isolated and stained by Alizarine Red S, then observed under stereomicroscope. Scanning electron microscopy (SEM) an

SUBMITTER: Pang L 

PROVIDER: S-EPMC7035872 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature