Rotation tracking of genome-processing enzymes using DNA origami rotors.

ABSTRACT: Many genome-processing reactions, including transcription, replication and repair, generate DNA rotation. Methods that directly measure DNA rotation, such as rotor bead tracking^1-3, angular optical trapping⁴ and magnetic tweezers⁵, have helped to unravel the action mechanisms of a range of genome-processing enzymes that includes RNA polymerase (RNAP)⁶, gyrase², a viral DNA packaging motor⁷ and DNA recombination enzymes⁸. Despite the potential of rotation measurements to transform our understanding of genome-processing reactions, measuring DNA rotation remains a difficult task. The time resolution of existing methods is insufficient for tracking the rotation induced by many enzymes under physiological conditions, a

SUBMITTER: Kosuri P 

PROVIDER: S-EPMC7036295 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature