ISX-9 manipulates endocrine progenitor fate revealing conserved intestinal lineages in mouse and human organoids.

ABSTRACT:

Objective

Enteroendocrine cells (EECs) survey the gut luminal environment and coordinate hormonal, immune and neuronal responses to it. They exhibit well-characterised physiological roles ranging from the control of local gut function to whole body metabolism, but little is known regarding the regulatory networks controlling their differentiation, especially in the human gut. The small molecule isoxazole-9 (ISX-9) has been shown to stimulate neuronal and pancreatic beta-cell differentiation, both closely related to EEC differentiation. Our aim was to use ISX-9 as a tool to explore EEC differentiation.

Methods

We investigated the effects of ISX-9 on EEC differentiation in mouse and human intestinal organoids, using real-time quantitative polymerase chain reaction (RT-qPCR), f

SUBMITTER: Tsakmaki A 

PROVIDER: S-EPMC7036449 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature