Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis.

ABSTRACT: Pathogenic yeasts Candida albicans and Candida parapsilosis possess a ß-type carbonic anhydrase Nce103p, which is involved in CO₂ hydration and signaling. C. albicans lacking Nce103p cannot survive in low CO₂ concentrations, e.g., in atmospheric growth conditions. Candida carbonic anhydrases are orthologous to the Saccharomyces cerevisiae enzyme, which had originally been detected as a substrate of a non-classical export pathway. However, experimental evidence on localization of C. albicans and C. parapsilosis carbonic anhydrases has not been reported to date. Immunogold labeling and electron microscopy used in the present study showed that carbonic anhydrases are localized in the cell wall and plasmatic membrane of bot

SUBMITTER: Dostal J 

PROVIDER: S-EPMC7036955 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature