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GC-NICI-MS analysis of acetazolamide and other sulfonamide (R-SO₂-NH₂) drugs as pentafluorobenzyl derivatives [R-SO₂-N(PFB)₂] and quantification of pharmacological acetazolamide in human urine.

ABSTRACT: Acetazolamide (molecular mass (MM), 222) belongs to the class of sulfonamides (R-SO₂-NH₂) and is one of the strongest pharmacological inhibitors of carbonic anhydrase activity. Acetazolamide is excreted unchanged in the urine. Here, we report on the development, validation and biomedical application of a stable-isotope dilution GC-MS method for the reliable quantitative determination of acetazolamide in human urine. The method is based on evaporation to dryness of 50 μL urine aliquots, base-catalyzed derivatization of acetazolamide (d₀-AZM) and its internal standard [acetylo-²H₃]acetazolamide (d₃-AZM) in 30 vol% pentafluorobenzyl (PFB) bromide in acetonitrile (60 min, 30 °C), reconstitution in toluene (200 μL) and inj

SUBMITTER: Begou O

PROVIDER: S-EPMC7037487 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Dataset Information

GC-NICI-MS analysis of acetazolamide and other sulfonamide (R-SO₂-NH₂) drugs as pentafluorobenzyl derivatives [R-SO₂-N(PFB)₂] and quantification of pharmacological acetazolamide in human urine.

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Dataset Information

GC-NICI-MS analysis of acetazolamide and other sulfonamide (R-SO2-NH2) drugs as pentafluorobenzyl derivatives [R-SO2-N(PFB)2] and quantification of pharmacological acetazolamide in human urine.

OmicsDI is part of the ELIXIR infrastructure

Tweets

GC-NICI-MS analysis of acetazolamide and other sulfonamide (R-SO₂-NH₂) drugs as pentafluorobenzyl derivatives [R-SO₂-N(PFB)₂] and quantification of pharmacological acetazolamide in human urine.