Genome-Wide Identification, Expression Profile and Evolution Analysis of Karyopherin ? Gene Family in Solanum tuberosum Group Phureja DM1-3 Reveals Its Roles in Abiotic Stresses.

ABSTRACT: In eukaryotic cells, nucleocytoplasmic trafficking of macromolecules is largely mediated by Karyopherin ?/Importin (KPN? or Imp?) nuclear transport factors, and they import and export cargo proteins or RNAs via the nuclear pores across the nuclear envelope, consequently effecting the cellular signal cascades in response to pathogen attack and environmental cues. Although achievements on understanding the roles of several KPN?s have been obtained from model plant Arabidopsis thaliana, comprehensive analysis of potato KPN? gene family is yet to be elucidated. In our genome-wide identifications, a total of 13 StKPN? (Solanum tuberosum KPN?) genes were found in the genome of the doubled monoploid S. tuberosum Group Phureja DM1-3. Sequence alignment and conserved domain analysis suggested the presence of importin-? N-terminal domain (IBN_N, PF08310) or Exporin1-like domain (XpoI, PF08389) at N-terminus and HEAT motif at the C-terminal portion in most StKPN?s. Phylogenetic analysis indicated that members of StKPN? could be classified into 16 subgroups in accordance with their homology to human KPN?s, which was also supported by exon-intron structure, consensus motifs, and domain compositions. RNA-Seq analysis and quantitative real-time PCR experiments revealed that, except StKPN?3d and StKPN?4, almost all StKPN?s were ubiquitously expressed in all tissues analyzed, whereas transcriptional levels of several StKPN?s were increased upon biotic/abiotic stress or phytohormone treatments, reflecting their potential roles in plant growth, development or stress responses. Furthermore, we demonstrated that silencing of StKPN?3a, a SA- and H2O2-inducible KPN? genes led to increased susceptibility to environmental challenges, implying its crucial roles in plant adaption to abiotic stresses. Overall, our results provide molecular insights into StKPN? gene family, which will serve as a strong foundation for further functional characterization and will facilitate potato breeding programs.

SUBMITTER: Xu Y 

PROVIDER: S-EPMC7037939 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature