Chimerization Enables Gene Synthesis and Lentiviral Delivery of Customizable TALE-Based Effectors.

ABSTRACT: Designer effectors based on the DNA binding domain (DBD) of Xanthomonas transcription activator-like effectors (TALEs) are powerful sequence-specific tools with an excellent reputation for their specificity in editing the genome, transcriptome, and more recently the epigenome in multiple cellular systems. However, the repetitive structure of the TALE arrays composing the DBD impedes their generation as gene synthesis product and prevents the delivery of TALE-based genes using lentiviral vectors (LVs), a widely used system for human gene therapy. To overcome these limitations, we aimed at chimerizing the DNA sequence encoding for the TALE-DBDs by introducing sufficient diversity to facilitate both their gene synthesis and enable their lentiviral delivery. To this end, we replaced three out

SUBMITTER: Fang Y 

PROVIDER: S-EPMC7038218 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature