Integration, abundance, and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines.

ABSTRACT: BACKGROUND:As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in a double-stranded break and repair at the targeted locus, oftentimes resulting in mutation(s) at the intended site. As soybean is a self-pollinating species with 20 chromosome pairs, the transgene(s) in the T0 plant are generally expected to be unlinked to the targeted mutation(s), and the transgene(s)/mutation(s) should independently assort into the T1 generation, resulting in Mendellian combinations of transgene presence/absence and allelic states within the segregating family. This prediction, however, is not always consistent with observed results. RESULTS:In this study

SUBMITTER: Michno JM 

PROVIDER: S-EPMC7038615 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature