A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics.

ABSTRACT:

Background

Myxospermy is a process by which the external surfaces of seeds of many plant species produce mucilage-a polysaccharide-rich gel with numerous fundamental research and industrial applications. Due to its functional properties the mucilage can be difficult to remove from the seed and established methods for mucilage extraction are often incomplete, time-consuming and unnecessarily wasteful of precious seed stocks.

Results

Here we tested the efficacy of several established protocols for seed mucilage extraction and then downsized and adapted the most effective elements into a rapid, small-scale extraction and analysis pipeline. Within 4 h, three chemically- and functionally-distinct mucilage fractions were obtained from myxospermous seeds. These fractions were used

SUBMITTER: Cowley JM 

PROVIDER: S-EPMC7038624 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature