A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea.

ABSTRACT: General molecular principles of ribosome biogenesis have been well explored in bacteria and eukaryotes. Collectively, these studies have revealed important functional differences and few similarities between these processes. Phylogenetic studies suggest that the information processing machineries from archaea and eukaryotes are evolutionary more closely related than their bacterial counterparts. These observations raise the question of how ribosome synthesis in archaea may proceed in vivo. In this study, we describe a versatile plasmid-based cis-acting reporter system allowing to analyze in vivo the consequences of ribosomal RNA mutations in the model archaeon Haloferax volcanii. Applying this system, we provide evidence that the bulge-helix-bulge motif enclosed within the ribosomal RNA pr

SUBMITTER: Juttner M 

PROVIDER: S-EPMC7038931 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature