Strong suppression of gene conversion with increasing DNA double-strand break load delimited by 53BP1 and RAD52.

ABSTRACT: In vertebrates, genomic DNA double-strand breaks (DSBs) are removed by non-homologous end-joining processes: classical non-homologous end-joining (c-NHEJ) and alternative end-joining (alt-EJ); or by homology-dependent processes: gene-conversion (GC) and single-strand annealing (SSA). Surprisingly, these repair pathways are not real alternative options restoring genome integrity with equal efficiency, but show instead striking differences in speed, accuracy and cell-cycle-phase dependence. As a consequence, engagement of one pathway may be associated with processing-risks for the genome absent from another pathway. Characterization of engagement-parameters and their consequences is, therefore, essential for understanding effects on the genome of DSB-inducing agents, such as ionizing-radiati

SUBMITTER: Mladenov E 

PROVIDER: S-EPMC7038941 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature