Differential expression of duplicated ribosomal protein genes modifies ribosome composition in response to stress.

ABSTRACT: In Saccharomyces cerevisiae, most ribosomal proteins are synthesized from duplicated genes, increasing the potential for ribosome heterogeneity. However, the contribution of these duplicated genes to ribosome production and the mechanism determining their relative expression remain unclear. Here we demonstrate that in most cases, one of the two gene copies generate the bulk of the active ribosomes under normal growth conditions, while the other copy is favored only under stress. To understand the origin of these differences in paralog expression and their contribution to ribosome heterogeneity we used RNA polymerase II ChIP-Seq, RNA-seq, polyribosome association and peptide-based mass-spectrometry to compare their transcription potential, splicing, mRNA abundance, translation potential, pr

SUBMITTER: Ghulam MM 

PROVIDER: S-EPMC7038994 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature