Structural basis of reiterative transcription from the pyrG and pyrBI promoters by bacterial RNA polymerase.

ABSTRACT: Reiterative transcription is a non-canonical form of RNA synthesis by RNA polymerase in which a ribonucleotide specified by a single base in the DNA template is repetitively added to the nascent RNA transcript. We previously determined the X-ray crystal structure of the bacterial RNA polymerase engaged in reiterative transcription from the pyrG promoter, which contains eight poly-G RNA bases synthesized using three C bases in the DNA as a template and extends RNA without displacement of the promoter recognition σ factor from the core enzyme. In this study, we determined a series of transcript initiation complex structures from the pyrG promoter using soak-trigger-freeze X-ray crystallography. We also performed biochemical assays to monitor template DNA translocation during RNA synthesis fr

SUBMITTER: Shin Y 

PROVIDER: S-EPMC7039003 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature