PTPN14 phosphatase and YAP promote TGFβ signalling in rheumatoid synoviocytes.

ABSTRACT:

Objective

We aimed to understand the role of the tyrosine phosphatase PTPN14-which in cancer cells modulates the Hippo pathway by retaining YAP in the cytosol-in fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA).

Methods

Gene/protein expression levels were measured by quantitative PCR and/or Western blotting. Gene knockdown in RA FLS was achieved using antisense oligonucleotides. The interaction between PTPN14 and YAP was assessed by immunoprecipitation. The cellular localisation of YAP and SMAD3 was examined via immunofluorescence. SMAD reporter studies were carried out in HEK293T cells. The RA FLS/cartilage coimplantation and passive K/BxN models were used to examine the role of YAP in arthritis.

Results

RA FLS displayed overexpression

SUBMITTER: Bottini A 

PROVIDER: S-EPMC7039277 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature