Genome editing retraces the evolution of toxin resistance in the monarch butterfly.

ABSTRACT: Identifying the genetic mechanisms of adaptation requires the elucidation of links between the evolution of DNA sequence, phenotype, and fitness¹. Convergent evolution can be used as a guide to identify candidate mutations that underlie adaptive traits^2-4, and new genome editing technology is facilitating functional validation of these mutations in whole organisms^1,5. We combined these approaches to study a classic case of convergence in insects from six orders, including the monarch butterfly (Danaus plexippus), that have independently evolved to colonize plants that produce cardiac glycoside toxins^6-11. Many of these insects evolved parallel amino acid substitutions in the α-subunit (ATPα) of the sodium pump (Na⁺/K⁺-ATPase

SUBMITTER: Karageorgi M 

PROVIDER: S-EPMC7039281 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature