The retina and retinal pigment epithelium differ in nitrogen metabolism and are metabolically connected.

ABSTRACT: Defects in energy metabolism in either the retina or the immediately adjacent retinal pigment epithelium (RPE) underlie retinal degeneration, but the metabolic dependence between retina and RPE remains unclear. Nitrogen-containing metabolites such as amino acids are essential for energy metabolism. Here, we found that ¹⁵N-labeled ammonium is predominantly assimilated into glutamine in both the retina and RPE/choroid ex vivo [¹⁵N]Ammonium tracing in vivo show that, like the brain, the retina can synthesize asparagine from ammonium, but RPE/choroid and the liver cannot. However, unless present at toxic concentrations, ammonium cannot be recycled into glutamate in the retina and RPE/choroid. Tracing with ¹⁵N-labeled amino acids show that the reti

SUBMITTER: Xu R 

PROVIDER: S-EPMC7039560 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature