EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD.

ABSTRACT: Sequential mannose trimming of N-glycan (Man₉GlcNAc₂ -> Man₈GlcNAc₂ -> Man₇GlcNAc₂) facilitates endoplasmic reticulum-associated degradation of misfolded glycoproteins (gpERAD). Our gene knockout experiments in human HCT116 cells have revealed that EDEM2 is required for the first step. However, it was previously shown that purified EDEM2 exhibited no α1,2-mannosidase activity toward Man₉GlcNAc₂ in vitro. Here, we found that EDEM2 was stably disulfide-bonded to TXNDC11, an endoplasmic reticulum protein containing five thioredoxin (Trx)-like domains. C558 present outside of the mannosidase homology domain of EDEM2 was linked to C692 in Trx5, which solely contains the CXXC motif in TXNDC11. This covalent b

SUBMITTER: George G 

PROVIDER: S-EPMC7039678 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature