Paradoxical network excitation by glutamate release from VGluT3⁺ GABAergic interneurons.

ABSTRACT: In violation of Dale's principle several neuronal subtypes utilize more than one classical neurotransmitter. Molecular identification of vesicular glutamate transporter three and cholecystokinin expressing cortical interneurons (CCK⁺VGluT3⁺INTs) has prompted speculation of GABA/glutamate corelease from these cells for almost two decades despite a lack of direct evidence. We unequivocally demonstrate CCK⁺VGluT3⁺INT-mediated GABA/glutamate cotransmission onto principal cells in adult mice using paired recording and optogenetic approaches. Although under normal conditions, GABAergic inhibition dominates CCK⁺VGluT3⁺INT signaling, glutamatergic signaling becomes predominant when glutamate decarboxylase (GAD) function is compromis

SUBMITTER: Pelkey KA 

PROVIDER: S-EPMC7039679 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature