Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria.

ABSTRACT: Dictyostelium discoideum is an intriguing model organism for the study of cell differentiation processes during development, cell signaling, and other important cellular biology questions. The technologies available to genetically manipulate Dictyostelium cells are well-developed. Transfections can be performed using different selectable markers and marker re-cycling, including homologous recombination and insertional mutagenesis. This is supported by a well-annotated genome. However, these approaches are optimized for axenic cell lines growing in liquid cultures and are difficult to apply to non-axenic wild-type cells, which feed only on bacteria. The mutations that are present in axenic strains disturb Ras signaling, causing excessive macropinocytosis required for feeding, and impair cel

SUBMITTER: Paschke P 

PROVIDER: S-EPMC7039707 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature