Improved filtration method to isolate pure populations of primary bovine endometrial epithelial and stromal cells for immunological studies.

ABSTRACT: OBJECTIVES:Isolation and culture of distinct primary endometrial cells are key to reliable in-vitro models to investigate the uterine immune response and optimse new disease interventions. Details on the isolation method and purity of distinct cell populations is lacking in currently available protocols leading to inconsistent results across laboratories. METHODS:Bovine endometrial tissue from non-pregnant bovine uteri were collected immediately post-mortem and separated using differential size filtering. Isolations (n = 15) yielded an average of 3.1 × 105 ± 0.7 × 105 epithelial cells and 1.88 × 106 ± 5.44 × 105 stromal fibroblasts per uterine horn. Following expansion in culture, the purity of cell populations was confirmed using morphology and positive staining for cytokeratin and viment

SUBMITTER: Kelly P 

PROVIDER: S-EPMC7039867 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature