Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii.

ABSTRACT: Beet cyst nematodes depend on a set of secretory proteins (effectors) for the induction and maintenance of their syncytial feeding sites in plant roots. In order to understand the relationship between the beet cyst nematode H. schachtii and its host, identification of H. schachtii effectors is crucial and to this end, we sequenced a whole animal pre-infective J2-stage transcriptome in addition to pre- and post-infective J2 gland cell transcriptome using Next Generation Sequencing (NGS) and identified a subset of sequences representing putative effectors. Comparison between the transcriptome of H. schachtii and previously reported related cyst nematodes and root-knot nematodes revealed a subset of esophageal gland related sequences and putative effectors in common across the tested species.

SUBMITTER: Elashry AM 

PROVIDER: S-EPMC7039985 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature