Theory and applications of differential scanning fluorimetry in early-stage drug discovery.

ABSTRACT: Differential scanning fluorimetry (DSF) is an accessible, rapid, and economical biophysical technique that has seen many applications over the years, ranging from protein folding state detection to the identification of ligands that bind to the target protein. In this review, we discuss the theory, applications, and limitations of DSF, including the latest applications of DSF by ourselves and other researchers. We show that DSF is a powerful high-throughput tool in early drug discovery efforts. We place DSF in the context of other biophysical methods frequently used in drug discovery and highlight their benefits and downsides. We illustrate the uses of DSF in protein buffer optimization for stability, refolding, and crystallization purposes and provide several examples of each. We also sho

SUBMITTER: Gao K 

PROVIDER: S-EPMC7040159 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature