Optimized expression and enhanced production of alkaline protease by genetically modified Bacillus licheniformis 2709.

ABSTRACT:

Background

Bacillus licheniformis 2709 is extensively applied as a host for the high-level production of heterologous proteins, but Bacillus cells often possess unfavorable wild-type properties, such as production of viscous materials and foam during fermentation, which seriously influenced the application in industrial fermentation. How to develop it from a soil bacterium to a super-secreting cell factory harboring less undomesticated properties always plays vital role in industrial production. Besides, the optimal expression pattern of the inducible enzymes like alkaline protease has not been optimized by comparing the transcriptional efficiency of different plasmids and genomic integration sites in B. licheniformis.

Result

Bacillus licheniformis 2709 was genetically modif

SUBMITTER: Zhou C 

PROVIDER: S-EPMC7041084 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature