Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance.

ABSTRACT:

Background

Differential expression of mucins has been associated with several cancers including colorectal cancer (CRC). In normal physiological conditions, secretory mucin MUC5AC is not expressed in the colonic mucosa, whereas its aberrant expression is observed during development of colon cancer and its precursor lesions. To date, the molecular mechanism of MUC5AC in CRC progression and drug resistance remains obscure.

Methods

MUC5AC expression was determined in colon tissue microarray by immunohistochemistry. A RNA interference and CRISPR/Cas9-mediated system was used to knockdown/knockout the MUC5AC in CRC cell lines to delineate its role in CRC tumorigenesis using in vitro functional assays and in vivo (sub-cutaneous and colon orthotopic) mouse models. Finally, CRC cell

SUBMITTER: Pothuraju R 

PROVIDER: S-EPMC7041280 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature