Effects of salt-loading on supraoptic vasopressin neurones assessed by ClopHensorN chloride imaging.

ABSTRACT: Salt-loading (SL) impairs GABA_A inhibition of arginine vasopressin (AVP) neurones in the supraoptic nucleus (SON) of the hypothalamus. Based on previous studies, we hypothesised that SL activates tyrosine receptor kinase B (TrkB), down-regulating the activity of K⁺ /Cl^- co-transporter2 (KCC2) and up-regulating Na⁺ /K⁺ /Cl^- co-transporter1 (NKCC1). These changes in chloride transport would result in increased [Cl^- ]_i in SON AVP neurones. The study combined virally-mediated chloride imaging with ClopHensorN with a single-cell western blot analysis. An adeno-associated virus with ClopHensorN and a vasopressin promoter (AAV2-0VP1-ClopHensorN) was bilaterally injected in the SON of adult male Sprague-Dawley

SUBMITTER: Balapattabi K 

PROVIDER: S-EPMC7041405 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature