Comparison of Three Commercial Tools for Metagenomic Shotgun Sequencing Analysis.

ABSTRACT: Metagenomic shotgun sequencing for the identification of pathogens is being increasingly utilized as a diagnostic method. Interpretation of large and complicated data sets is a significant challenge, for which multiple commercial tools have been developed. Three commercial metagenomic shotgun sequencing tools, CosmosID, One Codex, and IDbyDNA, were compared to determine whether they result in similar interpretations of the same sequencing data. We selected 24 diverse samples from a previously characterized data set derived from DNA extracted from biofilms dislodged from the surfaces of resected arthroplasties (sonicate fluid). Sequencing data sets were analyzed using the three commercial tools and compared to culture results and prior metagenomic analysis interpretation. Identical interpre

SUBMITTER: Thoendel M 

PROVIDER: S-EPMC7041569 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature