Mifamurtide and TAM-like macrophages: effect on proliferation, migration and differentiation of osteosarcoma cells.

ABSTRACT: Tumor-associated macrophages and their alternative activation states together with cytokines and growth factors trapped in tumor microenvironment contribute to the progression of OS. In contrast to other tumor types, M2 polarized macrophages, reduce metastasis and improve survival in osteosarcoma patients. Mifamurtide is an immunomodulatory drug given together with standard adjuvant chemotherapy in high-grade osteosarcoma to improve outcome. Macrophages obtained from peripheral blood mononucleated cells of healthy donors and MG63 cells were cultured alone and together, and treated with Mifamurtide. We analyzed the effects of Mifamurtide on macrophage polarization and on MG63 proliferation, migration and differentiation, evaluating the expression of M1/M2 and osteoblast markers and molecule

SUBMITTER: Punzo F 

PROVIDER: S-EPMC7041936 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature