DNA Sensors' Signaling in NK Cells During HHV-6A, HHV-6B and HHV-7 Infection.

ABSTRACT:

Objectives

The host DNA sensor proteins TLR9, STING, IFI16 are central signaling molecules that control the innate immune response to cytosolic nucleic acids. Here we propose to investigate how Natural killer (NK) cell infection by human herpesvirus (HHV)-6A, HHV-6B or HHV-7 is able to modify DNA sensor signaling in NK cells.

Methods

We infected the NK92 cell line and primary NK cells with cell-free inocula of HHV-6A, HHV-6B or HHV-7 and evaluated TLR9, STING, and IFI16 pathway expression by Real-Time PCR, Western Blot, immunofluorescence and flow cytometry for 1, 2, 3, and 6 days post-infection. We evaluated NK cell cytokine-producing by Real-Time PCR and enzyme immunosorbent assay.

Results

NK92 and primary NK cells were promptly infected by three viruses, as demonst

SUBMITTER: Bortolotti D 

PROVIDER: S-EPMC7042408 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature