Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients.

ABSTRACT: Sponge-associated bacteria possess biotechnologically interesting properties but as yet have largely evaded cultivation. Thus, "omics"-based information on the ecology and functional potential of sponge symbionts is awaiting its integration into the design of innovative cultivation approaches. To cultivate bacteria derived from the marine sponge Aplysina aerophoba, nine novel media formulations were created based on the predicted genomic potential of the prevalent sponge symbiont lineage Poribacteria. In addition, to maintain potential microbial metabolic interactions in vitro, a Liquid-Solid cultivation approach and a Winogradsky-column approach were applied. The vast majority of microorganisms in the inoculum appeared viable after cryopreservation of sponge specimen as dete

SUBMITTER: Gutleben J 

PROVIDER: S-EPMC7042410 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature