Midgut de novo transcriptome analysis and gene expression profiling of Spodoptera exigua larvae exposed with sublethal concentrations of Cry1Ca protein.

ABSTRACT: Spodoptera exigua (Hübner) is a polyphagous pest on agricultural crops, whose control is based mainly on the application of chemical insecticides. Bacillus thuringiensis (Bt) is one of the most important biological agents that have been successfully applied as a biological control, and Cry1Ca protein is considered to be active against S. exigua. Therefore, to understand the response of S. exigua to Cry1Ca protein, high-throughput sequencing was used to analyse the S. exigua larval midgut after treatment with sublethal concentrations of Cry1Ca protein. Transcriptome data showed that a total of 98,571 unigenes with an N50 value of 1135 bp and a mean length of 653 bp were obtained. Furthermore, 2962 differentially expressed genes (DEGs) were identified after

SUBMITTER: Ren X 

PROVIDER: S-EPMC7042451 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature