Handheld instrument for wound-conformal delivery of skin precursor sheets improves healing in full-thickness burns.

ABSTRACT: The current standard of care for patients with severe large-area burns consists of autologous skin grafting or acellular dermal substitutes. While emerging options to accelerate wound healing involve treatment with allogeneic or autologous cells, delivering cells to clinically relevant wound topologies, orientations, and sizes remains a challenge. Here, we report the one-step in situ formation of cell-containing biomaterial sheets using a handheld instrument that accommodates the topography of the wound. In an approach that maintained cell viability and proliferation, we demonstrated conformal delivery to surfaces that were inclined up to 45° with respect to the horizontal. In porcine pre-clinical models of full-thickness burn, we delivered mesenchymal stem/stromal cell-containing fibrin s

SUBMITTER: Cheng RY 

PROVIDER: S-EPMC7042907 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature