Ca²⁺ mobilization-dependent reduction of the endoplasmic reticulum lumen is due to influx of cytosolic glutathione.

ABSTRACT:

Background

The lumen of the endoplasmic reticulum (ER) acts as a cellular Ca²⁺ store and a site for oxidative protein folding, which is controlled by the reduced glutathione (GSH) and glutathione-disulfide (GSSG) redox pair. Although depletion of luminal Ca²⁺ from the ER provokes a rapid and reversible shift towards a more reducing poise in the ER, the underlying molecular basis remains unclear.

Results

We found that Ca²⁺ mobilization-dependent ER luminal reduction was sensitive to inhibition of GSH synthesis or dilution of cytosolic GSH by selective permeabilization of the plasma membrane. A glutathione-centered mechanism was further indicated by increased ER luminal glutathione levels in response to Ca²⁺ efflux. Inducible reducti

SUBMITTER: Lizak B 

PROVIDER: S-EPMC7043043 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature