Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.

ABSTRACT:

Background

Pancreatic organoid systems have recently been described for the in vitro culture of pancreatic ductal cells from mouse and human. Mouse pancreatic organoids exhibit unlimited expansion potential, while previously reported human pancreas organoid (hPO) cultures do not expand efficiently long-term in a chemically defined, serum-free medium. We sought to generate a 3D culture system for long-term expansion of human pancreas ductal cells as hPOs to serve as the basis for studies of human pancreas ductal epithelium, exocrine pancreatic diseases and the development of a genomically stable replacement cell therapy for diabetes mellitus.

Results

Our chemically defined, serum-free, human pancreas organoid culture medium supports the generation and expansion of hPOs with h

SUBMITTER: Georgakopoulos N 

PROVIDER: S-EPMC7043048 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature