The 5' NAD Cap of RNAIII Modulates Toxin Production in Staphylococcus aureus Isolates.

ABSTRACT: Nicotinamide adenosine dinucleotide (NAD) has been found to be covalently attached to the 5' ends of specific RNAs in many different organisms, but the physiological consequences of this modification are largely unknown. Here, we report the occurrence of several NAD-RNAs in the opportunistic pathogen Staphylococcus aureus Most prominently, RNAIII, a central quorum-sensing regulator of this bacterium's physiology, was found to be 5' NAD capped in a range from 10 to 35%. NAD incorporation efficiency into RNAIII was found to depend in vivo on the -1 position of the P3 promoter. An increase in RNAIII's NAD content led to a decreased expression of alpha- and delta-toxins, resulting in reduced cytotoxicity of the modified strains. These effects seem to be caused neither by changes

SUBMITTER: Morales-Filloy HG 

PROVIDER: S-EPMC7043667 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature