SwsB and SafA Are Required for CwlJ-Dependent Spore Germination in Bacillus subtilis.

ABSTRACT: When Bacillus subtilis spores detect nutrients, they exit dormancy through the processes of germination and outgrowth. A key step in germination is the activation of two functionally redundant cell wall hydrolases (SleB and CwlJ) that degrade the specialized cortex peptidoglycan that surrounds the spore. How these enzymes are regulated remains poorly understood. To identify additional factors that affect their activity, we used transposon sequencing to screen for synthetic germination defects in spores lacking SleB or CwlJ. Other than the previously characterized protein YpeB, no additional factors were found to be specifically required for SleB activity. In contrast, our screen identified SafA and YlxY (renamed SwsB) in addition to the known factors GerQ and CotE as proteins requir

SUBMITTER: Amon JD 

PROVIDER: S-EPMC7043669 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature