Defining the TLT-1 interactome from resting and activated human platelets.

ABSTRACT: The triggering receptor expressed on myeloid cells (TREM) protein family forms a class of type I transmembrane proteins expressed in immune cells that play important roles in innate and adaptive immune responses. The TREM family member TREM-like transcript 1 (TLT-1, also TREML1) is expressed in megakaryocytes and packaged into platelet granules. TLT-1 binds fibrinogen and plays a role in bleeding initiated by inflammatory insults. Here, we describe a proteomics screen that maps the TLT-1 interactome in resting and activated human platelets. Several identified TLT-1 interactors are involved in cell adhesion and migration, as well as platelet activation. Select interactors, including β₃-integrin, RACK1, GRB2, and Rabs 5A, 7, and 11A, were additionally characterized in co-immunopre

SUBMITTER: Schmoker AM 

PROVIDER: S-EPMC7044047 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature