Dinucleoside polyphosphates act as 5'-RNA caps in bacteria.

ABSTRACT: It has been more than 50 years since the discovery of dinucleoside polyphosphates (Np_nNs) and yet their roles and mechanisms of action remain unclear. Here, we show that both methylated and non-methylated Np_nNs serve as RNA caps in Escherichia coli. Np_nNs are excellent substrates for T7 and E. coli RNA polymerases (RNAPs) and efficiently initiate transcription. We demonstrate, that the E. coli enzymes RNA 5'-pyrophosphohydrolase (RppH) and bis(5'-nucleosyl)-tetraphosphatase (ApaH) are able to remove the Np_nN-caps from RNA. ApaH is able to cleave all Np_nN-caps, while RppH is unable to cleave the methylated forms suggesting that the methylation adds an additional layer to RNA stability regulation. Our work introduces a different perspect

SUBMITTER: Hudecek O 

PROVIDER: S-EPMC7044304 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature