Role of cell-type specific nucleosome positioning in inducible activation of mammalian promoters.

ABSTRACT: The organization of nucleosomes across functional genomic elements represents a critical layer of control. Here, we present a strategy for high-resolution nucleosome profiling at selected genomic features, and use this to analyse dynamic nucleosome positioning at inducible and cell-type-specific mammalian promoters. We find that nucleosome patterning at inducible promoters frequently resembles that at active promoters, even before stimulus-driven activation. Accordingly, the nucleosome profile at many inactive inducible promoters is sufficient to predict cell-type-specific responsiveness. Induction of gene expression is generally not associated with major changes to nucleosome patterning, and a subset of inducible promoters can be activated without stable nucleosome depletion from their tr

SUBMITTER: Oruba A 

PROVIDER: S-EPMC7044431 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature