Regulation of hepatic glutamine metabolism by miR-122.

ABSTRACT:

Objective

It is well established that the liver-specific miR-122, a bona fide tumor suppressor, plays a critical role in lipid homeostasis. However, its role, if any, in amino acid metabolism has not been explored. Since glutamine (Gln) is a critical energy and anaplerotic source for mammalian cells, we assessed Gln metabolism in control wild type (WT) mice and miR-122 knockout (KO) mice by stable isotope resolved metabolomics (SIRM) studies.

Methods

Six-to eight-week-old WT and KO mice and 12- to 15-month-old liver tumor-bearing mice were injected with [U-¹³C₅,¹⁵N₂]-L-Gln, and polar metabolites from the liver tissues were analyzed by nuclear magnetic resonance (NMR) imaging and ion chromatography-mass spectrometry (IC-MS). Gln-met

SUBMITTER: Sengupta D 

PROVIDER: S-EPMC7044666 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature