Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes.

ABSTRACT: The elucidation of biological processes involving reactive oxygen species (ROS) facilitates a better understanding of the underlying progression of non-communicable diseases. Fluorescent probes are a powerful tool to study various ROS and have the potential to become essential diagnostic tools. We have developed a series of coumarin fluorescent probes for the selective and sensitive detection of peroxynitrite (ONOO^-), a key ROS. Coumarin based probes exhibit good photostability, large Stokes shift and high quantum yields. The three ratiometric probes all contain a boronate ester motif for the detection of ONOO^- and a distinctive organelle targeting group. The study of ONOO^- generation in a particular organelle will allow more precise disease profiling. Henc

SUBMITTER: Weber M 

PROVIDER: S-EPMC7044669 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature